Transfection is a specialized technique for introducing exogenous genes into cells. With the deepening of research on gene and protein functions, transfection has become a basic method often involved in laboratory work. Transfection can be roughly divided into three categories: physically mediated, chemically mediated, and biologically mediated. Electroporation, microinjection and gene gun are examples of introducing genes into cells through physical methods; there are many chemically mediated methods, such as the classic calcium phosphate co-precipitation method, liposome transfection method, and various cationic substance-mediated methods; biologically mediated methods include the more primitive protoplast transfection and various virus-mediated transfection technologies that are now more common.
An ideal cell transfection method should have the advantages of high transfection efficiency and low cytotoxicity. Virus-mediated transfection technology is currently the most efficient method of transfection and has the advantage of very low cytotoxicity. However, viral transfection methods have complex preparation procedures and are often highly selective for cell types, making them difficult to popularize in general laboratories. Other physically and chemically mediated transfection methods have their own characteristics.
It should be pointed out that no matter which transfection technology is used, to obtain optimal transfection results, it may be necessary to optimize the transfection conditions. There are many factors that affect transfection efficiency, ranging from cell type, cell culture conditions and cell growth status to the operational details of the transfection method, all of which need to be considered. In addition, various analysis kits and transfection tools are required during the transfection process to help and evaluate the transfection effect, including biochemical tools, antibiotics, fluorescent reagents, fluorescent dyes, and gene expression tools. To this end, CD Bioparticles provides Assay Kits & Transfection Tools to help solve the following challenges.
The challenges you might meet:
- Lack of transfection systems with high cell adaptability and selectivity
- Transfection reagents or vectors may have toxic effects on cells, leading to cell damage, apoptosis or maladaptation.
- Lack of methods to evaluate transfection results after transfection
- There is obvious non-specific transfection
- Transfection efficiency may not be high enough, especially for certain difficult-to-transfect cell types
Assay Kits & Transfection Tools Key features:
Assay Kits & Transfection Tools Key benefits:
- By combining DNA or RNA with magnetic particles, directional guidance and transfection of cells can be achieved
- High transfection efficiency
- Specific reaction or combination with the target molecule generates a fluorescent signal, thereby achieving qualitative and quantitative analysis of the transfection effect
- Successfully transfected cells can be screened in batches using antibiotic genes
Assay Kits & Transfection Tools Application candidates:
- Suitable for transfection of difficult-to-transfect cell types, such as hematopoietic cells and neurons
- Can be used to improve the transfection efficiency of ordinary cells
- Can be used as antibiotic screening marker
- For tracking intracellular expression and distribution of transfected molecules
- Magnetic targeted transfection of target molecules into cells
