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Clipos™ pHrodo-Red-Dextran Labeled Phosphatidylglycerol (PG) Liposomes

Catalog:CDEDXF-019

Unit Size
Price
$2800

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Specifications QA & Reviews
StorageShould always be stored at in the dark at 4°C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE.
AppearanceColored translucent liquid made of encapsulated fluorescent dextran formulation, and the color depends on the type of the fluorescent dye that is used. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
DescriptionDextran is a branched polysaccharide with glucose subunits. It is a large molecule that over time cannot leak out of the liposome, which is located inside the vial. Dextran can be tagged with a variety of fluorescent probes. Liposomes encapsulating fluorescent dextran can be used in experiments that require tracking of fluorescently water-soluble cargo. Dextrans conjugated to dyes such as pHrodo-Red and pHrodo-Green are pH sensitive and increase in fluorescence as pH decreases from more neutral to acidic. The pHrodo-Red and pHrodo-Green conjugates are commonly used to detect endocytosis and phagocytosis, respectively, as the dyes exhibit little to no fluorescence outside the cell at neutral pH and, upon internalization, emit a bright red or green signal due to the acidic environment of the endosome and phagosome, respectively. The liposome formulation of encapsulated dextran fluorescent dyes is extensively dialyzed and cleaned, thus eliminating the need for the end user to separate encapsulated dextran fluorescent dyes from non-encapsulated ones.
Lipid compositionDOPG/DOPC/Cholesterol (20/50/30 molar ratio)
Liposome Size100 nm
Lipid Molar Concentration10 mM
FluorophorepHrodo-Red-Dextran
Excitation560 nm
Emission585 nm
Buffer20 mM HEPES, pH 7.4
Shelf-life30 days.
Technical NotesUnilamellar liposomes can be made in 50 nm and 200 nm sizes. Liposomes that are larger than 200 nm are multilamellar structure. Liposomes are made in HEPES buffer at pH 7.4 but it can be made in any other buffer of your choice. You can specify your buffer at the time of ordering. Liposomes are made in degassed buffer that is purged with argon to avoid oxidation of the unsaturated phospholipids. Liposomes are made under sterile condition. Liposomes are unilamellar and therefore due to their small size they will not settle in the bottom of the vial. If you need to take multiple aliquots out of the vial during a period of time, then it is advised to take extreme care in not contaminating the vial. It is recommended to handle the vial under a sterile hood to maintain the sterility of the product. Liposomes should never be frozen. Crystal ice that is formed during freezing will rupture the lipid membrane of the liposomes and change the size the liposomes particles.
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