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Clipos™ EGFP mRNA Lipid Nanoparticles

Catalog:CDPM01

Unit Size:

INQUIRY Datasheet

Specifications QA & Reviews
StorageStore at 4°C.
DescriptionThis product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of EGFP mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The lipid nanoparticles used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. EGFP is an enhanced version of GFP (green fluorescent protein), originally isolated from Aequorea Victoria, and shows a bright green fluorescence with an emission peak of 509nm. EGFP is a useful marker for tracking gene expression and transfection efficiency in mammalian cultures. The Sequence of EGFP used is available upon request. mRNA isolated in sodium citrate, sodium acetate, lipid mix in ethanol, and PBS.
Lipid compositionSM-102/DSPC/cholesterol/DMG-PEG2000
Emission509nm
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice. Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/ml] in a total of 1ml per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency. 2. Briefly pipette mRNA-LNP mix up and down to resuspend. Add 20ul of the mRNA-LNP mix dropwise directly to your 1ml culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker) Place your transfected cells back into their original culture conditions. 3. Check cell expression by FACS at 24hr intervals after transfection. *Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
Encoding mRNAControl EGFP-2
In Vitro QC (Cell Types)HEK293S, iPSC, T cells
Technology ApplicationsControl
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