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Clipos™ mCherry-Luciferase mRNA Lipid Nanoparticles

Catalog:CDPM03

Unit Size:

INQUIRY Datasheet

Specifications QA & Reviews
StorageStore at 4°C
DescriptionmCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching. The mRNA is capped using CleanCap, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. mRNA isolated in sodium citrate, sodium acetate, lipid mix in ethanol, and PBS.
Excitation587 nm
Emission610 nm
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice. Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/ml] in a total of 1ml per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency. 2. Briefly pipette mRNA-LNP mix up and down to resuspend. Add 20ul of the mRNA-LNP mix dropwise directly to your 1ml culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker) Place your transfected cells back into their original culture conditions. 3. Check cell expression by FACS at 24hr intervals after transfection. *Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
Encoding mRNAmCherry-Luciferase
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsControl
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