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Clipos™ COVID-19 Spike Protein (Alpha Variant) mRNA Lipid Nanoparticles

Catalog:CDPM11

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INQUIRY Datasheet

Specifications QA & Reviews
StorageStore at 4°C
DescriptionThis product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of the Alpha variant spike protein (COVID-19) mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The lipid nanoparticles used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. COVID-19 Spike Proteins are highly glycosylated and play a major role in penetrating and initiating infection in host cells by coronaviruses. Spike proteins utlize two subunits: S1 recognizes and binds to the host cell causing conformational change that will allow fusion of viral envelope and host cell membrane by S2. The Alpha variant of this spike protein arrived in late November 2020. It has an estimated 80% better transmission rate than its wild-type counterpart. This ability has rendered the Alpha variant spike protein a candid vice for mRNA delivery. mRNA encapsulated in lipid nanoparticle, primarily in PBS (-ca, -mg). Dilute levels of: sodium acetate, ethanol, free mRNA & free lipids.
Lipid compositionSM-102/DSPC/cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice. Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
Usage1. Prior to transfection, in a 12 well culture plate, plate your cells at [0.5E6 cells/mL] in a total of 1 mL per well. Ensure the cells you are using are viable and healthy. Try not to let your cells sit for longer than 5 minutes prior to transfection; cell clumping at the time of transfection may reduce transfection efficiency. 2. Briefly pipette mRNA-LNP solution up and down to resuspend. Add 20 uL of the mRNA-LNP mix dropwise directly to your 1 mL culture. Gently tilt plate back and forth to mix (not necessary if you are using cells which will be immediately placed back into a shaker). Place your transfected cells back into their original culture conditions. 3. Check cell expression by FACS at 24hr intervals after transfection. *Note: This is a generalized protocol for transfection using mammalian cells. Transfection volume may be scaled down/up proportionately.
Encoding mRNACOVID-19 Spike protein (Alpha variant)
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsVaccine development
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