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N¹-[4-(4,4'-Dimethoxytrityloxy)butyl]-N¹,N4,N9,N¹²-tetrakis(trifluoroacetyl)-spermine-N¹²-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite


N¹-[4-(4,4'-Dimethoxytrityloxy)butyl]-N¹,N4,N9,N¹²-tetrakis(trifluoroacetyl)-spermine-N¹²-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Spermine Phosphoramidite

Catalog:CDGLR09

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$680

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Common UseSpermine phosphoramidite is used to produce oligospermine-oligonucleotide conjugates - Zip Nucleic Acids Oligos. The conjugates are believed to use the oligospermine to seek out and move along (scan) oligonucleotide strands until the probe complementary sequence is located. The oligospermine then performs the function of stabilizing the formed duplex by reducing electrostatic repulsion, thereby leading to significantly increased binding affinities. Spermine phosphoramidite is simple to use in oligonucleotide synthesis and can be added multiple times at the 3' or 5' terminus. Deprotection and isolation are also straightforward. HPLC analysis of the conjugates requires high pH to suppress the ionization of the spermine residues.
Structure N¹-[4-(4,4'-Dimethoxytrityloxy)butyl]-N¹,N4,N9,N¹²-tetrakis(trifluoroacetyl)-spermine-N¹²-butyl-4-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
StorageFreezer storage, -10 to -30°C, dry
Molecular Weight1233.17
Molecular FormulaC56H73F12N6O9P
Stability1-2 days
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