Storage | Store at 4°C |
Description | This product is designed as a tool for the delivery and expression of HA-tagged CAS9 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of HA-tagged CAS9 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The sequence of the CAR9-HA protein consists of CAR9 containing 1368 amino acids and a C-terminal HA tag (YPYDVPDYA). mRNA encapsulated in lipid nanoparticle, primarily in PBS(-Ca, -Mg) (pH: 7.0-7.4). |
Lipid composition | SM-102/DSPC/Cholesterol/DMG-PEG2000 |
Technical Notes | Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended |
Encoding mRNA | Cas9-HA |
In Vitro QC (Cell Types) | HEK293S, T cells, iPSC |
Technology Applications | Control |
Product Name | Catalog | Lipid composition | Liposome Size | Price |
---|---|---|---|---|
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDL23-077-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1595 | |
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDL23-096-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1328 | |
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDL23-069-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1595 | |
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDMB01 | INQUIRY | ||
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDPM15 | SM-102/DSPC/cholesterol/DMG-PEG2000 | INQUIRY | |
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDPM03 | INQUIRY | ||
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDL23-082-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1695 | |
Clipos™ Cas9-HA mRNA Lipid Nanoparticles | CDSNT81 | 40-200 nm | $580 |
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2. Enter product information on the template (maximum number of products: 200).
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