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Clipos™ Oct-4 mRNA Lipid Nanoparticles

Catalog:CDL23-021-L

Unit Size

200 μL

Price: $1595

Buy Now Datasheet

Specifications QA & Reviews

Storage	Store at 4°C
Description	OCT-4 (Octamer-binding transcription factor 4) is a transcription factor protein of the POU (Pit-Oct-Unc) family, encoded by the POU5F1 gene. The transcription factor Oct-4 is capable of inducing an embryonic stem cell-like state, and it works together with Sox2, Klf4, and usually c-Myc to generate induced pluripotent stem cells (iPSCs). These factors are commonly referred to as "Yamanaka reprogramming factors," and this reprogramming effect reverts human fibroblasts to iPSCs. In addition, Oct-4 plays a crucial role in determining the fate of endoplasmic cells and embryonic stem cells, with the function of maintaining the pluripotency of embryonic cells or regulating the proliferation of cancer cells. This product is designed as a tool for the delivery and expression of OCT-4 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of OCT-4 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The OCT-4 protein is about 40 kD and consists of 360 amino acids
Lipid composition	SM-102/DSPC/Cholesterol/DMG-PEG2000
Technical Notes	Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNA	POU5F1
In Vitro QC (Cell Types)	HEK293S
Technology Applications	Protein research

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