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Clipos™ LIN28 mRNA Lipid Nanoparticles


Catalog:CDL23-024-L

Unit Size
Price
$1595

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StorageStore at 4°C
DescriptionLIN28 is an evolutionarily highly conserved RNA-binding protein that functions as an oncogene in higher eukaryotes. LIN28 regulates diverse physiological functions such as development, differentiation, insulin resistance and tumorigenesis. Overexpression of human LIN28A and its paralog LIN28B is associated with many tumors, leading to markedly increased cancer aggressiveness and poor prognosis. LIN28 has been reported to be overexpressed in many cancers, implying that LIN28 is an excellent biomarker for many tumors, including prostate cancer. This product is designed as a tool for the delivery and expression of LIN28A mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of LIN28A mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The LIN28A protein is about 23 kD and consists of 209 amino acids
Lipid compositionSM-102/DSPC/Cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNALIN28
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsProtein research
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