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Clipos™ C-Myc mRNA Lipid Nanoparticles

Catalog:CDL23-025-L

Unit Size
Price
$1595

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Specifications QA & Reviews
StorageStore at 4°C
DescriptionMyc is a family of regulatory genes and proto-oncogenes encoding transcription factors. The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL) and n-myc (MYCN). c-myc (sometimes also called MYC) was the first gene identified in this family because of its homology to the viral gene v-myc. The transcription factor C-myc is a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. C-myc regulates the transcription of specific target genes by forming heterodimers with the related transcription factor MAX and binding the E-box DNA consensus sequence. In cancer, c-myc is often constitutively expressed, leading to increased expression of many genes, some of which are involved in cell proliferation and contribute to cancer development. There is evidence that C-myc translation can be initiated from upstream out-of-frame AUG (CUG) and downstream AUG initiation sites, resulting in two isoforms with distinct N-termini. This product is designed as a tool for the delivery and expression of C-myc mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of C-myc mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The C-Myc protein is about 50 kD and consists of 454 amino acids
Lipid compositionSM-102/DSPC/Cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNAC-Myc
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsProtein research
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