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Clipos™ CD40-TF tag mRNA Lipid Nanoparticles

Catalog:CDL23-042-L

Unit Size
Price
$1595

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Specifications QA & Reviews
StorageStore at 4°C
DescriptionCD40 (Cluster of differentiation 40) is a type I transmembrane protein encoded by the CD40 gene and belongs to the TNF receptor superfamily. CD40 is a co-stimulatory receptor molecule, which is mainly expressed on antigen-presenting cells, some non-immune cells and tumors, and binds to CD40 ligand molecules transiently expressed on T cells and non-immune cells under inflammatory conditions. CD40/CD40L signaling acts as an immune checkpoint, controlling and modulating host immune responses to various diseases and pathogens, as well as the development of cancer and autoimmune diseases. Due to its essential role in immune activation, the CD40/CD40L interaction is considered an attractive target for immunotherapy. This product is designed as a tool for the delivery and expression of TF-tagged human CD40 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hCD40-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hCD40-TF protein is about 33 kD, consisting of hCD40 and a C-terminal TF tag (15 amino acids)
Lipid compositionSM-102/DSPC/Cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNACD40
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsProtein research
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