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Clipos™ IFN-gamma-TF tag mRNA Lipid Nanoparticles

Catalog:CDL23-045-L

Unit Size

200 μL

Price: $1595

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Specifications QA & Reviews

Storage	Store at 4°C
Description	Interferon gamma (IFN-γ or IFN-Gamma), encoded by the IFNG gene, is a dimerized soluble cytokine that is the only member of the type II interferon class. IFN-γ is secreted by cells of the innate and adaptive immune systems. It forms homodimers (active form) that bind to the IFN-γ receptor and trigger cellular responses to viral and microbial infections. Mutations in the IFN-γ gene are associated with increased susceptibility to viral, bacterial and parasitic infections, as well as several autoimmune diseases. IFN-γ has been reported to prevent tumor growth, suggesting that this type II interferon could be used in the development of cancer immunotherapies. This product is designed as a tool for the delivery and expression of TF-tagged human IFN-γ mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIFNγ-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hIFNγ-TF protein is about 20 kD, consisting of hIFNγ and a C-terminal TF tag (15 amino acids)
Lipid composition	SM-102/DSPC/Cholesterol/DMG-PEG2000
Technical Notes	Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNA	IFNG
In Vitro QC (Cell Types)	HEK293S
Technology Applications	Protein research

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