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Clipos™ IL-7-TF tag mRNA Lipid Nanoparticles

Catalog:CDL23-054-L

Unit Size
Price
$1980

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Specifications QA & Reviews
StorageStore at 4°C
DescriptionInterleukin 7 (IL-7) is a hematopoietic growth factor encoded by the human IL7 gene. It plays a vital role in health maintenance and disease prevention, and congenital defects in IL-7 signaling lead to severe immune deficiency. IL-7, a molecule known for its growth-promoting effects on B-cell progenitors, remains one of the most widely studied cytokines. IL-7 stimulates the differentiation of pluripotent hematopoietic stem cells into lymphoid progenitors and the proliferation of all cells of the lymphoid lineage (B cells, T cells and NK cells). Clinical trials of recombinant IL-7 as an immunotherapeutic agent for various malignancies have demonstrated its safety and effective immune reconstitution. This product is designed as a tool for the delivery and expression of TF-tagged human IL-7 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIL7-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The IL7-TF protein is about 21 kD, consisting of hIL7 and a C-terminal TF tag (15 amino acids)
Lipid compositionSM-102/DSPC/Cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNAIL7
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsProtein research
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