Storage | Store at 4°C |
Description | Interleukin 15 (IL-15), encoded by the IL-15 gene, is a member of the family of four alpha-helical bundles of cytokines. The primary mechanism of action of IL-15 appears to be juxtacrine signaling determined by cell-cell contacts. This role also includes endocrine and reverse signaling. IL-15 was initially characterized as a soluble molecule, but it was later found that the major form of IL-15 protein is the membrane-bound form, which can be directly bound to the cell membrane or presented by the IL-15Rα receptor. The IL-15 receptor consists of three subunits: IL-15Rα, CD122, and CD132. CD122 and CD132 are shared with the IL-2 receptor, and there is actually an additional subunit (CD25). The shared subunits contain cytoplasmic motifs required for signal transduction that underlie many of the overlapping biological activities of IL-15 and IL-2. IL-15Rα is a subunit that specifically binds IL-15 with very high affinity and can bind IL-15 independently of other subunits. This property suggests that IL-15 is able to transmit signals between multiple cells. This product is designed as a tool for the delivery and expression of IL-15-IL15Rα mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of IL-15-IL15Rα mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The IL-15-IL15Rα fusion protein is approximately 59 kD, consisting of full-length human IL-15 (162 amino acids) and IL15Rα (353 amino acids) with 15-residue peptide linker GGGGSGGGGSGGGGS between them |
Lipid composition | SM-102/DSPC/Cholesterol/DMG-PEG2000 |
Technical Notes | Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended |
Encoding mRNA | IL-15 |
In Vitro QC (Cell Types) | HEK293S |
Technology Applications | Protein research |
Product Name | Catalog | Lipid composition | Liposome Size | Price |
---|---|---|---|---|
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-095-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1328 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-047-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1595 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-056-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1595 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-067-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1980 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDSNT80 | $1380 | ||
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-036-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1595 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDL23-090-L | SM-102/DSPC/Cholesterol/DMG-PEG2000 | $1980 | |
Clipos™ IL-15-IL15Ralpha mRNA Lipid Nanoparticles | CDPM09 | SM-102/DSPC/cholesterol/DMG-PEG2000 | INQUIRY |
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2. Enter product information on the template (maximum number of products: 200).
3. Load the file using selector below.