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Clipos™ eGFP-T2A-mCherry mRNA Lipid Nanoparticles


Catalog:CDL23-096-L

Unit Size
Price
$1328

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StorageStore at 4°C
DescriptionGreen Fluorescent Protein (GFP) is a protein that fluoresces bright green when illuminated by light in the blue to ultraviolet range. eGFP is a GFP that produces enhanced green fluorescence and is widely used as a reporter gene in cellular and molecular biology. mCherry is a protein of the monomeric red fluorescent protein (mRFP) mFruits family, derived from DsRed of the Discosoma sea anemone. mCherry is a fluorescent protein chromophore used as a tool to visualize genes and analyze their function in experiments. mCherry absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. This product is designed as a tool for the delivery and expression of both eGFP and mCherry using eGFP-T2A-mCherry mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of eGFP-T2A-mCherry mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. eGFP-T2A-mCherry mRNA-LNP produces eGFP and mCherry separated by a T2A cleavage peptide
Lipid compositionSM-102/DSPC/Cholesterol/DMG-PEG2000
Technical NotesWork with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice.Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended
Encoding mRNAeGFP-T2A-mCherry
In Vitro QC (Cell Types)HEK293S
Technology ApplicationsProtein research
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