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EXO293F-Exosome Standard


EXO293F-Exosome Standard

Catalog:CDE24-088-L

Unit Size:

INQUIRY Datasheet

StorageStore at -80°C
AppearanceLiquid
DescriptionIt is an ultra-high purity exosome pellet obtained from HEK293F cells of clear origin, after obtaining the supernatant by serum-free culture, and then separated and purified by Tangential Flow Ultrafiltration (TFF) and Density Gradient Centrifugation (DGC), which can be used for exosome characterization standards or controls, etc.Exosomes purity: ≥5E+11 Particles/mg
Shelf-lifeHalf year
Technical Notes1. Avoid repeated freezing and thawing, due to the inherent properties of exosomes, the number of particles will decrease with the prolongation of the preservation time, so it is necessary to use as soon as possible. 2. Although exosome protection agent can improve the stability of exosome preservation, but will affect the protein concentration detection (BCA), it is recommended to use the factory protein concentration directly. If you need to re BCA quantification, you need to change the sample with phosphate buffer and other laboratory routine buffer, and then protein concentration measurement.
Technology ApplicationsStandards or controls for exosome characterization, including but not limited to: exosome morphology observation (transmission electron microscopy, TEM), particle size distribution detection (nanofluidic, NanoFCM; or Nanoparticle Tracking Analysis, NTA), exosome marker assay (Western Blot, WB), zeta potential determination, and other research standards or controls.
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