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ExoLoad-Exosomal Modification LTH-Lipid-Anchor Kit #2

ExoLoad-Exosomal Modification LTH-Lipid-Anchor Kit #2

Catalog:CDE24-097-L

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Structure ExoLoad-Exosomal Modification LTH-Lipid-Anchor Kit #2
StorageStore according to instructions
DescriptionThis kit contains LTH-FITC target peptide molecules polymerized with PEG cholesterol. Cholesterol is an amphiphilic substance with both lipophilic and hydrophilic groups, but its lipophilicity is stronger than hydrophilicity, and it can be embedded between phospholipid molecules when interacting with phospholipids, connecting the target molecules to the exosome external surface and realizing the surface modification of the exosome.LTH (LTHVVWL) is a target peptide obtained by phage screening technology that can target kidney injury molecule-1 (Kim-1), and is commonly used in the acute and chronic diseases. LTH (LTHVVWL) is a target peptide obtained by phage screening that can target the kidney injury molecule-1 (Kim-1), which is commonly used in the study of acute kidney injury, and FITC is a green fluorescent molecule with an excitation light of 488 nm and an emission light of 525 nm, which is chemically linked together to attach a green fluorescent-labeled LTH target peptide to the surface of the exosome, which is an excellent modification tool for the modification of the surface of the exosome.
Shelf-life1 year
Technical Notes1. Avoid repeated freezing and thawing. Due to the inherent properties of the lipid-anchored targeting peptide, it will agglomerate with the prolongation of storage time, please use it as soon as possible after thawing. 2. The kit comes with non-sterile ultrafiltration tubes (there are no sterile ultrafiltration tubes in the market), it is recommended to add antibiotics or further filtration to remove bacteria when ultrafiltrating the product for subsequent experiments, and the ultrafiltration tubes can be stored at room temperature. 3. It is recommended to operate in the ultra-clean table to avoid the breeding of bacteria by opening the cap repeatedly.
Composition• LTH-Lipid-Anchor Solution* 5 mg/1 tube • Dilution Buffer* 5 mL/1 bottle • Reaction Buffer* 40 mL/1 bottle • Wash Buffer* 250 mL/3 bottle
Technology Applications1. Co-incubation modification: Directly using co-incubation to connect target peptide molecules to obtain engineered exosomes with targeted effects. 2. Disease direction: research on the functional mechanism of functional molecule delivery in acute kidney injury and other related diseases.
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