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ExoLoad-Exosomal Modification IMTP-Lipid-Anchor Kit #1

ExoLoad-Exosomal Modification IMTP-Lipid-Anchor Kit #1

Catalog:CDE24-098-L

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Structure ExoLoad-Exosomal Modification IMTP-Lipid-Anchor Kit #1
StorageStore according to instructions
DescriptionThis kit contains IMTP-FAM targeting peptide molecules polymerized with PEG cholesterol. Cholesterol is an amphiphilic substance with both lipophilic and hydrophilic groups, but its lipophilicity is stronger than hydrophilicity, and it can be embedded between the phospholipid molecules when interacting with phospholipids, connecting the target molecules it carries with it on the external surface of the exosome to realize the surface modification of the exosome.IMTP (ischemic myocardium-targeting peptide, CSTSMLKAC) is a molecule obtained by phage screening technology that can target the deficient peptide. IMTP (ischemic myocardium-targeting peptide, CSTSMLKAC) is a targeting peptide obtained by phage screening technology that can target ischemic myocardial tissues, which is commonly used in ischemic heart disease related research.FAM is a green fluorescent molecule, with excitation light of 488 nm and emission light of 525 nm.By chemically linking the three molecules together, IMTP targeting peptide with green fluorescent markers can be attached to the surface of exosome and is an excellent modification tool for exosome surface modification. It is an excellent tool for exosome surface modification.
Shelf-life1 year
Technical Notes1. Avoid repeated freezing and thawing. Due to the inherent properties of the lipid-anchored targeting peptide, it will agglomerate with the prolongation of storage time, please use it as soon as possible after thawing. 2. The kit comes with non-sterile ultrafiltration tubes (there are no sterile ultrafiltration tubes in the market), it is recommended to add antibiotics or further filtration to remove bacteria when ultrafiltrating the product for subsequent experiments, and the ultrafiltration tubes can be stored at room temperature. 3. It is recommended to operate in the ultra-clean table to avoid the breeding of bacteria by opening the cap repeatedly.
Composition• IMTP-Lipid-Anchor Solution* 1 mg/1 mL/1 tube • Reaction Buffer* 5 mL/1 tube • Wash Buffer* 150 mL/1 bottle
Technology Applications1. Co-incubation modification: directly using co-incubation to connect target peptide molecules to obtain engineered exosomes with targeting effects. 2. Disease direction: research on the functional mechanism of functional molecule delivery in ischemic heart disease and other related diseases.
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