Short Name | ExoLoad-Exosomal Modification VHP-Lipid-Anchor Kit #1 |
Description | High throughput, can obtain the amount of targeted exosomes needed for in vivo experiments. Two-step operation, hands-on time of 30min can be completed exosome targeting peptide connection, the highest single flux up to, and efficiently solve the non-immortalized cells difficult to modify, pre-modification process of various technical difficulties, 293F exosome test positive rate of up to 70% or more. |
Technology Applications | 1. Co-incubation modification: directly using co-incubation to connect target peptide molecules to obtain engineered exosomes with targeting effects. 2. Disease direction: research on the functional mechanism of functional molecule delivery in atherosclerosis, ischemia/reperfusion injury, hypertension and other vascular diseases with high VCAM1 expression. |
Storage | Store according to instructions |
Shelf-life | 1 year |
Technical Notes | 1. Avoid repeated freezing and thawing. Due to the inherent properties of the lipid-anchored targeting peptide, it will agglomerate with the prolongation of storage time, please use it as soon as possible after thawing. 2. The kit comes with non-sterile ultrafiltration tubes (there are no sterile ultrafiltration tubes in the market), it is recommended to add antibiotics or further filtration to remove bacteria when ultrafiltrating the product for subsequent experiments, and the ultrafiltration tubes can be stored at room temperature. 3. It is recommended to operate in the ultra-clean table to avoid the breeding of bacteria by opening the cap repeatedly. |
Composition | • VHP-Lipid-Anchor Solution* 1 mg/1 mL/1 tube • Reaction Buffer* 5 mL/1 tube • Wash Buffer* 150 mL/1 bottle |
Structure |
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