Structure | |
Functional Group | Biotin, NHS |
Purity | 98% |
Storage | -20°C protected from light and moisture |
Molecular Weight | 504.7 |
Molecular Formula | C22H33N5O7S3 |
CAS No. | 142439-92-7 |
Appearance | White powder |
Description | Biotin disulfide-N-hydroxysuccinimide ester.Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate. NHS-SS-Biotin is an amine-reactive biotinylation reagent that contains a reducible disulfide. NHS-SS-Biotin is cell membrane per-meable and has a long chain separating the biotin group from the amino-reactive NHS-ester N-Hydroxysuccinimide ester. This product is often used to purify an antibody or other protein from a biotin specific affinity resin like immobilized streptavidin without exposing your protein of interest to denaturing conditions. Biotinylation reagent incorporating a spacer with a cleavable disulfide linkage. Typically coupled to primary amine in the pH range 6.5-8.5. Cleavage of disulfide requires reducing agent allowing mild recovery of biotinylated compound. |
Shelf-life | 1 year |
Solubility | Soluble in DMSO |
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