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DBCO-PEG4-DesthioBio


DBCO-PEG4-DesthioBio

Catalog:CDPB-04421

INQUIRY Datasheet

Common UseDBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of Biotin that binds to streptavidin with nearly equal specificity but less affinity than Biotin (Kd = 1011 vs. 1015M, respectively). Consequently, Desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free Biotin. For pull-down experiments with biological samples, this soft-release characteristic of Desthiobiotin also helps to minimize co-purification of endogenous Biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free Biotin.
Structure DBCO-PEG4-DesthioBio
PEG Molecular Weight719.9 g/mol
Functional GroupDBCO/Biotin
Purity95%
Storage-20°C
Molecular FormulaC39H53N5O8
CAS No.2032788-37-5

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