Common Use | DBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of Biotin that binds to streptavidin with nearly equal specificity but less affinity than Biotin (Kd = 1011 vs. 1015M, respectively). Consequently, Desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free Biotin. For pull-down experiments with biological samples, this soft-release characteristic of Desthiobiotin also helps to minimize co-purification of endogenous Biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free Biotin. |
Structure | |
PEG Molecular Weight | 719.9 g/mol |
Functional Group | DBCO/Biotin |
Purity | 95% |
Storage | -20°C |
Molecular Formula | C39H53N5O8 |
CAS No. | 2032788-37-5 |
Catalog: CDPB-04570
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