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Clipos™ DiO labeled Phosphatidylcholine (PC) Liposomes


Catalog:CDL6001F-DO

Unit Size
Price
$980

Buy Now Need technical support? Datasheet

Storage2-8 °C
DescriptionThe fluorescent control liposomes contain the green lipophilic dye DiO incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc. The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.
Lipid compositionDOPC/Cholesterol (54/45 molar ratio)
Liposome Size100 nm (90-120 nm)
Lipid Molar Concentration50 mM (50-55 mM)
FluorophoreDiO
Fluorophore Concentration0.5 mM (0.44 mg/mL)
Excitation484 nm
Emission501 nm
Buffer10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Shelf-life6 months guaranteed for unopened vials.
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