Storage | 2-8 °C |
Description | High quality 300nm LUV liposomes labeled with fluorescein DHPE are ready to use. The liposomes are prepared by the extrusion method to 300nm with tight particle size distribution. The product is semisterile filtered through 0.45um filters and filled in autoclaved vials guaranteed to be stable for 3 months stored in refrigerator. The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ~496/519 nm). For more information on the dye, see Invitrogen F-362. |
Lipid composition | DOPC/Cholesterol/F-DHPE (54/45/1 molar ratio) |
Liposome Size | 280 nm (250-320 nm) |
Lipid Molar Concentration | 50 mM (50-55 mM) |
Fluorophore | Fluorscein-DHPE |
Fluorophore Concentration | 0.5 mM (0.59 mg/mL) |
Excitation | 496 nm |
Emission | 519 nm |
Buffer | 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2 |
Shelf-life | 3 month for unopened vials, protect from light |
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