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Clipos™ Saturated Phosphatidylcholine (PC) Liposomes, Lyophilized

Catalog:CDELHP-1228

Unit Size
Price
$2100

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Specifications QA & Reviews
StorageShould always be stored in a freezer at at -40 °C or -80 °C. Upon adding water to proliposomes, liposomes are formed.
AppearanceWhite lyophilized powder. Liposomes are formed upon adding water to the freeze dried proliposomes and the appearance of the formulation will be white translucent liquid.
Lipid composition14/0 PC (DMPC)/Cholesterol (70/30 molar ratio)
Liposome Size100 nm (after hydration)
BufferLyophilized Phosphate Buffered Saline, 7.4 (after hydration)
Shelf-life6 months.
Technical NotesLiposomes are formed upon hydration of the lyophilized formulation. If the lyophilized liposomes are hydrated with solution containing a water soluble drug then a large percentage of the drug will stay outside of the liposomes and in non-encapsulated form. It is advised to use a micro dialysis cassette or a spin column using the right side beads (depending on the size of your drug) and separate the drug encapsulated liposomes from free drug and perform the drug assay in order to calculate the encapsulation efficiency. Lyophilized liposomes are mainly recommended to be used with drugs that have a short life in aqueous solution mainly due to hydrolysis. After adding the solution of the drug to lyophilized liposomes, the liposomes should be used immediately. Lyophilized Liposomes products should be used by scientists who understand liposome formulation and have the proper equipment in order to check the size, separate the non-encapsulated drug and do the proper assays. Trehalose is used as a lyoprotectant in all freeze dried liposome formulation. The size distribution after hydration of the freeze dried formulation will be around 100 nm.
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