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Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes

Catalog:CDECPC-1598

Unit Size

5 mL

Price: $2100

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Specifications QA & Reviews

Storage	Should always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, that the size of the liposomes will also change upon freezing and thawing.
Appearance	White translucent liquid made of nano size unilamellar liposomes. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
Description	Liposomes are extensively used to study the interaction of proteins, peptides and other molecules with the surface of a lipid membrane. One of the parameters that affects this interaction is the charge of the liposomal membrane. Liposomes are always made in aqueous environment and they are sized to the desired size in liquid state using various methods such as high pressure extrusion through nano sized pore track etch membranes. Liposome without water is meaningless. In rare occasions, liposomes are freeze dried and proliposomes are formed in the presence of a lyoprotectant such as trehalose. Using a lyoprotectant is necessary in order to maintain the size of the liposomes after rehydration. Natural PC-based Liposomes are composed of many liposomes made from phosphatidylcholine (PC) lipids that are made from natural lipids that are extracted from egg yolk and soy bean and also tissues and organs such as brain, heart and liver with and without cholesterol. Adding 30% cholesterol makes the liposomes serum stable and is necessary when the liposomes are used in the presence of serum.
Lipid composition	Brain PC/Cholesterol (70/30 molar ratio)
Liposome Size	100 nm
Lipid Molar Concentration	10 mM
Buffer	Phosphate Buffered Saline, pH 7.4
Shelf-life	4 months.
Technical Notes	Liposomes are unilamellar and sized to 100 nm. If you need them to be made in another size then you should mention that at the time of ordering. Unilamellar liposomes can be made in 50 nm and 200 nm sizes. Liposomes that are larger than 200 nm are multilamellar structure. Liposomes are made in PBS buffer at pH 7.4 but it can be made in any other buffer of your choice. You can specify your buffer at the time of ordering. Liposomes are made in degassed buffer that is purged with argon to avoid oxidation of the unsaturated phospholipids. Liposomes are made under sterile condition. Liposomes are unilamellar and therefore due to their small size they will not settle in the bottom of the vial. If you need to take multiple aliquots out of the vial during a period of time, then it is advised to take extreme care in not contaminating the vial. It is recommended to handle the vial under a sterile hood to maintain the sterility of the product. Liposomes should never be frozen. Crystal ice that is formed during freezing will rupture the lipid membrane of the liposomes and change the size the liposomes particles.

Related Products

Product Name	Catalog	Lipid composition	Liposome Size	Price
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDELPCG-1244	DSPC/Cholesterol/PEG2000-DSPE (65/30/5 molar ratio)	100 nm (after hydration)	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDELHP-1230	16/0 PC (DPPC)/Cholesterol (70/30 molar ratio)	100 nm (after hydration)	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDECUP-1537	100% 18/1 (Δ6-Cis) PC	100 nm	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDELPC-1273	Egg PC/Cholesterol (70/30 molar ratio)	100 nm (after hydration)	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDECPC-1594	100% Egg PC	100 nm	$2450
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDELPC-1272	Brain PC/Cholesterol (70/30 molar ratio)	100 nm (after hydration)	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDECDEE-1610	100% 12/0 Diether PC	100 nm	$2100
Clipos™ Natural Phosphatidylcholine (PC) Lipid Liposomes	CDECPC-1596	100% Liver PC	100 nm	$2100

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