Storage | 2-8 °C |
Description | High quality LUV liposomes labeled with Oregon Green? 488 DHPE (OG-DHPE) are ready to use. The liposomes are prepared by the extrusion method to 100 nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months when stored in refrigerator.The product is sterile filtered and filled in sterile vials guaranteed to be stable for 3 months when stored in refrigerator. The phospholipid, Oregon Green? 488 DHPE is labeled on the head group with the green-fluorescent Oregon Green? fluorophore with excitation/emission maxima ~496/524 nm. This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range. |
Lipid composition | DOPC/Cholesterol/OG-DHPE (54/45/1 molar ratio) |
Liposome Size | 100 nm (90-120 nm) |
Lipid Molar Concentration | 50 mM (50-55 mM) |
Fluorophore | Oregon Green 488-DHPE |
Fluorophore Concentration | 0.5 mM (0.54 mg/mL) |
Excitation | 496 nm |
Emission | 524 nm |
Buffer | 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2. |
Shelf-life | 6 month for unopened vials, protect from light. |
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