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Clipos™ FITC-Dextran labeled Phosphatidylglycerol (PG) Liposomes

Catalog:CDEFDC-1749

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INQUIRY Datasheet

Specifications QA & Reviews
StorageShould always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. ENS is not responsible for results generated by frozen product.
AppearanceYellow translucent liquid. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
DescriptionFluorescent leakage assay is a method that is extensively used for measuring the disruption of the liposomal membrane due to various external triggers such as interaction of molecules (e.g. peptides, proteins, detergents and etc.) with the surface of the liposomes. Liposomal membrane can also be disrupted when external energy is given to the system such as exposure of liposomes to sonic sound, mechanical pressure or heat. When liposomes are disrupted, their content will leak out of the liposomes. If you investigate the old literature, you will realize that in a large percentage of fluorescent leakage assay, the experiments involves encapsulating a self quenching concentration of fluorescent dyes such as calcein and carboxyfluorescein. The problem with calcein and carboxyfluorescein is that the liposomes have to be made very fresh and be used right away. These molecules have a tendency to leak out quickly in the absence of any trigger especially when unsaturated lipids are used. This will cause the assay to be less sensitive and the data not to be reproducible. These days, a very large selection of fluorescent dyes are commercially available which can be encapsulated or incorporated into the lipid bilayers of the liposomes.Fluorescent dextran molecules are excellent candidates for leakage assay. Dextran molecules can be tagged with various self quenching fluorophores such as FITC. Dextran molecules comes in different sizes and therefore they are very ideal candidates for studying the size of the pore that is formed on the liposome membrane. They can also be used for studies that involve the total collapse of liposomal structure as well.The approximate Stokes’ radii for FITC-dextrans are given below. Molecular Weight (MW) of Dextran (Dalton) Corresped to Approximate Stokes’ Radius (Angstrom): 4,000 MW (14 A), 10,000 MW (23 A), 20,000 MW (33 A), 40,000 MW (45 A), 70,000 MW (60 A), 150,000 MW (85 A) For example, if you observe a leakage using 4,000 dextran-FITC and you do not detect any leakage using 10K dextran FITC then it simply shows that the radius of the pore is larger than 14 Å but smaller than 23 Å.If your experiment involves the total collapse of the structure of the liposomes then any size dextran-FITC can be used.Unlik calcein, dextran-FITC does not leak out of the liposomes due to its size.
Lipid compositionDOPC/DOPG (60/40 molar ratio)
Liposome Size100 nm
FluorophoreFITC-Dextran
Excitation490 nm
Emission520 nm
BufferPhosphate Buffered Saline, pH 7.4
Shelf-life60 days
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