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Clipos™ Control of Doxorubicin Encapsulated Liposomes, Non PEGylated

Catalog:CDEDOX-1011

Unit Size
Price
$780

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Specifications QA & Reviews
Functional GroupPlain
StorageShould always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.
AppearanceThe product is a white translucent liquid made of nano size unilamellar liposomes. Usually, due to the small size of liposomes, no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
DescriptionFor most surface reactive liposome formulations, the reactive lipid is incorporated into the liposomes and the ligand, protein or peptide is conjugated directly to the liposomes that contain reactive lipids. However, this strategy might not work if the reactive lipid is prone to hydrolysis such as maleimide or NHS lipid. In order to get around this issue, the liposomes are made without the PEGylated (reactive and non-reactive) lipids. The scientist who is planning to use the kit will conjugate the ligand, protein or peptide to the reactive lipid. Next, the buffer is added to the lipid-conjugated ligand and micelle solution is formed. Another micelle solution is formed after the PEGylated non-reactive lipid is hydrated with the buffer. The two micelle solutions are mixed and incubated together at a temperature above the liquid-to-gel phase transition temperature of the HSPC (saturated matrix lipid). The PEGylated lipids in the micelles insert themselves with a high efficiency (above 80%) to the liposomes and form the immunoliposomes.
Lipid compositionHSPC/Cholesterol (60/40 molar ratio)
Liposome Size100 nm
Lipid Molar Concentration24.61 mM
Shelf-life4 months.
Lipid Mass Concentration15.33 mg/mL
Inside BufferAmmonium Sulfate, pH 7.4
Outside BufferPhosphate Buffered Saline, pH 7.4
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