×
Shopping Cart
×
Quote List
0Quotes
0Cart

Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated

Catalog:CDEIMF-1470

Unit Size
Price
$2100

Buy Now Need technical support? Datasheet

Specifications QA & Reviews
Functional GroupCyanur
StorageShould always be stored at in the dark at 4 °C, except when brought to room temperature for brief periods prior to animal dosing. DO NOT FREEZE. If the suspension is frozen, the encapsulated drug can be released from the liposomes thus limiting its effectiveness. In addition, the size of the liposomes will also change upon freezing and thawing.
AppearanceBe colored and the color is depend on the type of the fluorescent dye that is used. Usually due to the small size of liposomes no settling will occur in the bottom of the vial. The liposomes are packaged in an amber vial.
DescriptionProteins can be covalently coupled to the liposomes via amine-reactive cyanur-groups, either directly to the vesicle surface using cyanuric chloride-activated DSPE (cyanur-DSPE) or to the distal ends of PEG-spacers using activated cyanur-PEG-PE (ammonium salt). Cyanuric chloride at the PEG terminus functions to link peptides, antibodies and other amine-containing biomolecules or nanoparticles via a nucleophilic substitution reaction under basic conditions. Antibodies or other proteins can be conjugated without any previous derivatization.
Lipid compositionHSPC/Cholesterol/DSPE-PEG(2000)/DSPE-PEG(2000)-Cyanur (~57/38/4/1 molar ratio)
Liposome Size100 nm
Lipid Molar Concentration~21.58 mM
FluorophorePyrene-DOPE
Excitation351 nm
Emission379 nm
BufferPhosphate Buffered Saline, pH 7.4
Shelf-life2 months.
Technical NotesTris buffer should never be used in any step of the process since it contains amine. Cyanuric chloride is considered as a sensory respiratory irritant. However, despite the name of the cyanur-modified liposomes, they have not shown any sign of acute, chronic or genotoxicity. Cyanur groups are amine-reactive, however, some random attachments of the antibodies can be expected since cyanuric chloride can react with a wide range of nucleophilic functionalities, such as alcohols and thiols. This may interfere with the binding of the antibody to the liposome, and therefore, the binding affinity would change. Size exclusion spin columns such as Sepharose? CL-4B can be used instead of Float-A-Lyzer? dialysis cassette. However a very large amount of liposomes will stick to the column during the cleanup process and therefore we strongly suggest using dialysis than size exclusive beads. If you are using a ligand or peptide that is hydrophobic, it is recommended to solubilize it in DMSO or DMF and then add the buffer to it. It is recommended not to use more than 5% volume of DMSO or DMF in the solution. DMF and DMSO are both compatible with liposomes and they are also miscible in water. Other organic solvent such as ethanol and chloroform are not compatible with liposomes and will cause the liposomes to lyse. If you end up using DMSO or DMF then after the conjugation reaction is done, you need to remove DMSO and DMF from the liposomes. In order to do that you need to use a dialysis cassette that is made from REGENERATED CELLULOSE MEMBRANE. NOTE: Not all membranes are compatible with DMF and DMSO. We recommend using a Slide-A-Lyzer? MINI Dialysis Device with MWCO of 2K made from regenerated cellulose membrane manufactured by ThermoFisher. After DMSO or DMF is removed, you can use Float-A-Lyzer? dialysis device for the final step of cleaning up the prep.
Lipid Mass Concentration~15.92 mg/mL
My Review for Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated

Required fields are marked with *

Overall Rating *
×
Related Products
Product Name Catalog Lipid composition Liposome Size Price
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEFPC-1842 L-α-PC/Cholesterol/ 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(dibenzocyclooctyl) (69/30/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEIMF-1343 HSPC/Cholesterol/DSPE-PEG(2000)/DSPE-PEG(2000)-Folate (~57/38/4.5/0.5 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEIMF-1379 L-a-PC/Cholesterol/1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(6-azidohexanoyl) (~69/30/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEFDP-1817 DOTAP/DOPC/Cholesterol/1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(dibenzocyclooctyl) (5/64/30/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEFPS-1850 L-α-phosphatidylserine/L-α-PC/Cholesterol/ 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(dibenzocyclooctyl) (20/49/30/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEFDP-1887 DOTAP/DOPC/Cholesterol/ 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(6-azidohexanoyl) (ammonium salt) (5/64/30/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEIMF-1462 HSPC/Cholesterol/DSPE-PEG(2000)/DSPE-PEG(2000)-Cyanur (~57/38/4/1 molar ratio) 100 nm $2100
Clipos™ Pyrene labeled Liposomes, Cyanur Lipid, PEGylated CDEIMF-1504 L-a-PC/Cholesterol/1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine-N-(succinyl) (~69/30/1 molar ratio) 100 nm $2100
Products
Fill out the form below
to receive a quote

GET A QUOTE

Services

Drug Targeting Strategy

Drug Delivery Nanoparticles Formulation

Bioparticles Analysis and Characterization

Bioactive Particles & Fillers Production

Functional Biomedical Coatings

Lipid Library Development for Drug Delivery

Aptamer Targeted Drug Delivery System

Other Types of Drug Delivery

Products

Liposome System

Chelating Agents

Bioactive Matrixes

Porous Organic Materials

Biopolymers & Synthetic Polymers

PROTAC

Transfection

Exosome Products

Cleavable Linkers

Pharmaceutical Excipients (drug/medical grade)

Tissue Engineering Scaffold

  • (USA)
  • (Europe)
Cookie Policy | Privacy Policy | Copyright © CD Bioparticles. All rights reserved.
×
Upload products to cart

1. Download the template.

2. Enter product information on the template (maximum number of products: 200).

3. Load the file using selector below.

Drag and drop your file here or

Choose file
*.xlsx (max 20 MB)

4. Click the "Add to cart" button.

×
Upload products to quote list

1. Download the template.

2. Enter product information on the template (maximum number of products: 200).

3. Load the file using selector below.

Drag and drop your file here or

Choose file
*.xlsx (max 20 MB)

4. Click the "Add to quote" button.