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Standard 6-well Plates, 2 kPa

Standard 6-well Plates, 2 kPa

Catalog:CDAD37-3

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StorageRoom Temperature
DescriptionThis Standard plate comes with 5 standard 6-well plates, which is a tool to culture cell on PDMS substrates with various rigidity covering a broad physiological range, including 0.2, 0.5, 2, 8, 16, 32, and 64 kPa. The silicone gels are activated and ready to bind to a purified ECM, such as Type I Bovine Atelocollagen Solution (#CDAD59) prior to cell addition. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each well that is fully compatible with mammalian cell cultures. The surfaces of the gels in Standard plates are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells. The silicone substrates of Standard plates are optically clear and have a low auto-florescence. The layer of silicone in each well is firmly bonded to the bottom of the well. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods. Standard plates accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a plate. This product is for R&D use only and is not intended for human or other uses.
Shelf-lifeMinimum of 6 months from date of receipt.
FormPlate
Growth Area9.5 cm² growth area per well
Sterilization MethodOzone
Typical Working Volume2.0-3.5 mL per well
Cell Attachment AssayPass
Plate Surface MaterialFunctionalized Silicone
Elastic Modulus2
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