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Standard 6-well Plates Discovery Kit

Standard 6-well Plates Discovery Kit

Catalog:CDAD37-8

Unit Size
Price
$695

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StorageRoom Temperature
DescriptionThis kit provides you with 1 plate of each stiffness, which is a tool to culture cells on PDMS substrates with various elastic moduli of approximately 0.2, 0.5, 2, 8, 16, 32 and 64 kPa in 7 individual 6-well plates, allowing you to test how your cells behave across a broad range of physiologically relevant stiffness. The silicone gels are activated and ready to bind to a purified ECM, such as Type I Bovine Atelocollagen Solution (#CDAD59) prior to cell addition. The plates are packaged individually and sterilized, provided with 7 dishes per package. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each dish that is fully compatible with mammalian cell cultures. The surfaces of the gels in Standard plates are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells. The silicone substrates of products are optically clear and have a low auto-florescence. The layer of silicone in each dish is firmly bonded to the bottom of the dish. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods. Standard plates accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment, and there are no residuals of the substrate in the sample retrieved from a plate. This product is for R&D use only and is not intended for human or other uses.
Shelf-lifeMinimum of 6 months from date of receipt.
FormPlate
Growth Area9.5 cm2 growth area per well
Sterilization MethodOzone
Typical Working Volume2.0-3.5 mL per well
Cell Attachment AssayPass
Plate Surface MaterialFunctionalized Silicone
Elastic Modulus0.2, 0.5, 2, 8, 16, 32, 64 kPa (exact values provided on the CofA)
User Manual_Standard 6-well Plates Discovery Kit Download
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