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Methacrylated Collagen with Ruthenium Kit

Methacrylated Collagen with Ruthenium Kit

Catalog:CDAD40

Unit Size
Price
$850

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Specifications QA & Reviews
Purity>99% (Silver Staining)
Storage2-10°C
DescriptionA methacrylated Type I collagen kit. These 3D collagen gels can be prepared at various concentrations and photocrosslinked (400-450nm) to provide various gel stiffness. Each kit contains methacrylated collagen, ruthenium photoinitiator, acetic acid and a neutralization solution. The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes. A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Ruthenium and Sodium Persulfate to be formulated in 1x PBS or cell culture media which allows visible light photocrosslinking of the collagen at 400-450 nm. We recommend using antibiotics with the photoinitiator, or filtering through a 0.2 micron button filter for cell culture work. This product is for R&D use only and is not intended for human or other uses.
Shelf-lifeMinimum of 6 months from date of receipt. 2 month after reconstitution.
FormLyophilized Powder
Sterilization MethodFiltration
SourceBovine Hide
Electrophoretic PatternCharacteristic (Coomassie Blue)
Endotoxin<10.0 EU/mL (LAL)
SterilityNo growth (USP)
Extraction MethodAcid-Telocollagen
Gel Formation Tube Test<40 min
Hydrogel Young's Modulus ECharacteristic
Kinetic Gel Test<40 min
Component• Methacrylated Collagen, 100 mg • 20 mM Acetic Acid, 50 mL • Neutralization Solution, 10 mL • Ruthenium Photoinitiator, 100 mg • Sodium Persulfate Photoinitiator, 500 mg
Degree of Methacrylation>20%
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