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Thiol Dextran-3CG Hydrogel Kit (Slow Gelling)

Thiol Dextran-3CG Hydrogel Kit (Slow Gelling)

Catalog:CDAS019

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DescriptionThe Thiol Dextran-3CG Hydrogel Kit provides reagents for setting up hydrogels for 3-D cell culture or related applications. Its major components are Thiol Dextran and the crosslinker SCG-Link. When the two reagents are combined, thiol groups on 3CG-Link form stable thioether bonds with SH-reactive groups on SH-Dextran. The resulting polymer network forms a hydrogel. The crosslinking step can be performed in the presence of cells to embed cells in the hydrogel. Prior to the crosslinking step, cell adhesion peptides (e.g. RGD Peptide, Cat. No. CDAS012) can be covalently attached to a portion of the SH-reactive groups on Thiol Dextran to provide a cell-adhesive matrix. The formation of hydrogels is performed at physiological pH for optimal cell compatibility. Gelation starts several minutes after mixing the components at room temperature, providing enough time to conveniently manipulate the solution before the onset of gel formation. A matrix metalloprotease (MMP)-cleavable peptide in 3CG-Link with a broad range for MMP cleavage including MMP1, MMP3, MMP7 and MMP9* allows cells to locally degrade the polymer network, if they produce the indicated MMPs. This allows cells to spread and migrate within the hydrogel. In most cases cell spreading and migration also requires the presence of adhesion peptides. Thiol Dextran hydrogels can be dissolved by the addition of dextranase (Dextranase, Cat. No. CDAS016), which allows the recovery of chemically fixed or live cells after culture for post-culture analyses (e.g. RTPCR) or for further cultivation. Allows formation of up to 2 ml 3D Cell Culture Hydrogel depending on the grade of stiffness of the gel. Intended for research use only. Not for use in human therapeutic or diagnostic applications.
ReconstitutionBring lyophilized 3CG-Link: to room temperature. Briefly centrifuge vial to make sure that the entire material is at the bottom of the centrifuge tube. Add 188 µL water for a concentration of 20 mmol/L thiol groups. This will result in a 200 µL 3CG-Link solution. Close centrifuge tube and vortex briefly. Wait 5 min or until all material is dissolved. Briefly vortex and centrifuge again.
FormLyophilized Powder/Solution
Sterilization MethodFiltration
Component• Thiol-Dextran, 170 µL • 3CG-Link, lyophilized, 200 µL after reconstitution • 10 x CB (pH 7.2), 200 µL • Water, 2 x 1500 µL
Applications Note3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
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