Engineering mammalian cell surfaces with nanoparticles is a rapid, easy, and broadly applicable method for remodeling cell chemistry to introduce unnatural functions. CD Bioparticles is dedicated to cutting-edge nanotechnology research. We have created an advanced nanoparticle bioconjugation platform that includes custom synthesized protein-nanoparticle conjugates, peptide-nanoparticle conjugates, nucleic acid-nanoparticle conjugates, carbohydrate-nanoparticle conjugates, enzymes - Nanoparticle conjugates, virus-nanoparticle conjugates, liposome-nanoparticle conjugates services, and subsequent product formulation development and related characterization services.
Cell surface engineering is a unique technology at its core that personalizes the cell surface to modulate cell function and interactions. Cell surfaces constitute a highly diverse microenvironment, exhibiting a wide variety of protein, polysaccharide, and lipid components. These structures have critical roles in manipulating cell fate, including regulation of cell-cell interactions, communication of cells with their ecological environment, and modulation of intracellular signaling pathways. The cell surface has different bioactive functional groups, such as amine, sulfhydryl and carbonyl, which can modify the cell surface and provide a variety of target options for the introduction of exogenous functions.
Figure 1. Stable conjugation of nanoparticles (NPs) to the surfaces of T cells and HSCs via cell-surface thiols. (Matthias T Stephan, et al.; 2010)
Since the cell surface is closely related to the function of the cell, the use of polymer nanoparticles to construct the cell membrane to regulate the biochemical characteristics and functions of the cell provides new opportunities for the research of drug delivery, cell therapy, blood transfusion, tissue engineering and basic cell biology. The fusion of cells and polymers can improve in vivo compatibility, reduce immune response and enzymatic degradation, which brings a new perspective to the application fields of basic research of cell biology, blood transfusion, cell therapy and tissue engineering.
Covalent Modification of Living Cells
Although the cell surface has a variety of functional groups, only a small number of functional groups can react in the complex and heterogeneous environment of the cell surface. Among them, lysine (amine, -NH2) and cysteine (sulfhydryl, -SH) side chains are the most commonly used residues for direct covalent attachment of polymers to cell surfaces. This modification method is considered simple and straightforward as the cells do not need to undergo any pretreatment. However, exogenous ligation substrates require pre-activation prior to delivery to the cell surface. With rich experience in nanoparticle modification, CD Bioparticles has developed a highly integrated platform for the covalent coupling of living cells to polymers with different functional groups. Our covalent modification strategies include:
Cell Surface Induced Aggregation
Initiation of polymer synthesis via the cell surface offers unique advantages over proteins in terms of altering the physical properties of the modified surface, introducing secondary interacting functional groups, and providing the opportunity to generate cell-polymer hybrid structures. CD Bioparticles is dedicated to providing flexible polymer conjugation strategies to living cells at competitive prices. At CD Bioparticles, we provide a wide range of cell bioconjugation services (including red blood cells, white blood cells (lymphocytes, macrophages), stem cells (pluripotent and pluripotent), islet cells, cancer cells and endothelial cells) to global clients through A comprehensive and advanced platform to accelerate new drug discovery.
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