BIOORG MED CHEM LETT. 2022; 62, DOI:10.1016/j.bmcl.2022.128635

Evaluation of pentaerythritol-based and trimethylolpropane-based cationic lipidic materials for gene delivery

Wu, S; Liu, MY; Hu, X; He, CX; Zhao, CY; Xiang, SL; Zeng, YL

Abstract

The chemical and physical structure of cationic liposomes pays an important effect on their gene transfection efficiency. Investigation on the structure-function relationship of cationic liposomes will guide the design of novel cationic liposomes with high transfection efficiency and biosafety. In this paper, two novel series of lipids based on the backbone of pentaerythritol and trimethylolpropane were discovered, and their gene transfection efficiencies were assayed in vitro. The four lipids 8c, 9c, 14b, and 15b, exhibited much better transfection ef-ficiency in the HEK293 cell lines compared with Lipo2000, lipid 9c also showed good transfection efficiency in the SW480 cell lines. And the structure-efficiency relationship revealed that a hydroxyethyl polar head group boosted transfer potency in trimethylolpropane-type lipids, but reduced in pentaerythritol-type lipids.

Keywords: Gene delivery; Cationic lipids; Transfection efficiency; Structure-efficiency relationship

Related products/services

Lipids

Pentaerythritol-based and trimethylolpropane-based cationic lipidic materials are emerging as effective vectors for gene delivery, leveraging their unique structural properties to enhance transfection efficiency. These lipidic materials possess multiple hydroxyl groups, allowing for the formation of stable, compact lipoplexes with nucleic acids. Their cationic nature facilitates the interaction with negatively charged genetic material, promoting cellular uptake and endosomal escape. Pentaerythritol-based lipids offer increased branching, which enhances payload capacity, while trimethylolpropane-based lipids provide flexibility in modifying lipid tail lengths to optimize delivery efficiency. These versatile cationic lipids are crucial in advancing non-viral gene delivery systems, offering a promising alternative for therapeutic applications, including gene therapy and vaccine development.

Product Name	Catalog	Unit Size	Price
Clipos™ Cationic DOTMA Liposomes	CDEGEN-1695	2 mL	INQUIRY
Clipos™ Cationic GL-67 Liposomes	CDEGEN-1692	2 mL	INQUIRY
Lipid 8	CDL23-001L	100 mg, 500 mg	INQUIRY
Lipid 5	CDL23-002L	100 mg, 500 mg	INQUIRY
ALC-0315	CDL23-003L	100 mg, 500 mg	INQUIRY
1,17-bis(3-pentyloctyl) ester	CDL23-004L	100 mg, 500 mg	INQUIRY
DLin-MC3-DMA	CDL23-005L	100 mg, 1 g, 5 g	INQUIRY
13C3 Labelled-MC3	CDL23-006L	2 mg, 10 mg	INQUIRY
13C Labelled-Lipid 5	CDL23-007L	2 mg, 10 mg	INQUIRY
ATX-001	CDL23-008L	100 mg	INQUIRY
ATX-002	CDL23-009L	100 mg	INQUIRY
L319	CDL23-010L	25 mg, 50 mg, 100 mg	INQUIRY
DLin-KC3-DMA	CDL23-011L	100 mg, 250 mg, 1 g	INQUIRY
L322	CDL23-012L	100 mg, 500 mg	INQUIRY
DLin-MC2-DMA	CDL23-013L	100 mg, 1 g	INQUIRY
DLin-KC2-DMA	CDL23-014L	50 mg, 100 mg, 250 mg	INQUIRY
C12-200	CDL23-015L	25 mg, 50 mg, 100 mg	INQUIRY
CKK-E12	CDL23-016L	25 mg, 100 mg	INQUIRY
1,2-dioleoyl-sn-glycero-3-succinate	CDA24-091-L	100 mg, 500 mg	INQUIRY
1,2-dipalmitoyl-sn-glycero-3-succinate	CDA24-092-L	100 mg, 500 mg	INQUIRY

Prev :Bio-Inspired Coordination-Assembled Nanoparticles for Enhanced Drug Delivery of Tumor Chemotherapeutics

Next :Efficient Transfection of Large Plasmids Encoding HIV-1 into Human Cells-A High Potential Transfection System Based on a Peptide Mimicking Cationic Lipid

USA

Fill out the form below
to receive a quote

GET A QUOTE