As a promising drug carrier, liposomes require strict quality control for clinical use. In the previous article, we have introduced the requirements and methods of liposome morphology and particle size control. This article will introduce other parameters involved in liposomes as drug carriers，including: encapsulation rate, oxidation index, lysophospholipid, drug content, leakage rate, osmotic pressure and sterilization conditions. Encapsulation Efficiency Encapsulation efficiency is an important indicator to measure the quality of liposomes. It refers to the ratio of the encapsulated drug to the total amount of drug in the liposome suspension. The key to determining the encapsulation efficiency is to separate the unencapsulated free drug from the liposome suspension without destroying the…

As a drug carrier, liposome not only improves the in vivo behavior of the drug, improves the therapeutic index of the drug, reduces the toxicity of the drug, and reduces the adverse reactions of the drug, and has a certain slow-release and controlled-release effect, reduces the dose or frequency of drug administration, and improves patient compliance . According to the characteristics of liposome formulations, the stability of liposomes has always been a concern of researchers. Liposomes are easy to aggregate, drugs are easy to leak (decrease in encapsulation rate) and oxidative deterioration will directly affect the safety and effectiveness of liposome drugs, so the quality of liposome drugs should be studied and…

Liposomes have attracted researchers’ attention because of their unique properties. Its successful application as a drug delivery carrier makes it a key research object in the field of drug delivery. There are already many methods for the preparation of liposomes, the above has introduced thin film method, reverse evaporation method, re-emulsion method and centrifugation method. Immediately above, this article focuses on the injection method, calcium fusion method, ammonium sulfate gradient method, and high-pressure homogenization. Injection Method Dissolve lipids in water-miscible or immiscible organic solvents, inject into the aqueous phase with a micro syringe at an appropriate rate, and remove organic solvents by ultrafiltration, dialysis, reverse osmosis, reduced-pressure evaporation, etc. as needed.…

Phospholipids are generally white or light yellow powders or lumps at room temperature. They are very soluble in chloroform and can also be dissolved in ether, n-hexane or ethanol, and are almost insoluble in water or acetone. Disperse the phospholipid molecules into the aqueous solution by hydration treatment after high dispersion, or changing the solvent. When the concentration reaches the critical micelle concentration (CMC), self-assembly occurs through the hydrophobic interaction to form a bilayer structured liposome bubble. Commonly used preparation methods of liposomes in the laboratory include thin film method, reverse evaporation method, double emulsion method, centrifugal method, injection method, calcium fusion method, ammonium sulfate gradient method, etc. In order to ensure the final smooth transition to…