The technique of introducing exogenous genetic material into cells is a transfection technique commonly used in biological research. The key point of this method is the transfection efficiency, and the important parameter that limits the transfection efficiency in the entire transfection process is the amount of exogenous genetic material entering the cell.
To increase this number, researchers have tried various methods to optimize the transfection process, such as optimizing transfection conditions, using new transfection methods, and adding transporter uptake reagents. Among them, carrier uptake reagents provide a simple and effective strategy to improve transfection efficiency. This reagent can improve the uptake of foreign genetic material by cells to a certain extent and improve transfection efficiency.
CD Bioparticles' services with customized transfection strategies, precise designs and modifications of transfection carriers, and advanced technical platforms can help you to solve:
The challenges you might meet:
- The cell membrane prevents foreign genetic material from effectively entering the cell, resulting in low transfection efficiency.
- Foreign genetic material is easily degraded during transfection
- The introduction of foreign aid substances triggers cellular stress responses, resulting in unreliable or difficult reproducibility of experimental results.
- High transfection costs
Transporter Uptake Reagents Key Features:
Transporter Uptake Reagents Key Benefits:
- Transporter uptake reagents will increase the uptake efficiency of exogenous DNA or RNA into cells, thereby increasing the success rate of transfection.
- Some traditional transfection reagents can have toxic effects on cells, and using specially designed transporter uptake reagents may help mitigate this toxicity and improve cell health.
- Different transporter uptake reagents have higher specificity for specific types of cells or tissues, thereby ensuring that foreign molecules are more accurately taken up by target cells and reducing non-specific uptake.
- The use of transporter uptake reagents can simplify experimental procedures, which can help improve experimental reproducibility and efficiency.
- Low immune rejection
- Suitable for in vivo experiments
- Ready-to-use
ransporter Uptake Reagents Application Candidates:
- Gene transfer: Transporter uptake reagent is commonly used to facilitate entry of foreign genes (such as DNA in an expression vector) into target cells.
- This is a common application in fields such as gene therapy, gene expression, and protein expression.
- Delivery of siRNA and miRNA: Carrier uptake reagent is also widely used in RNAi research to achieve gene silencing and regulation by promoting small interfering RNA (siRNA) or microRNA (miRNA) into cells.
- Protein delivery: Some transporter uptake reagents can facilitate protein entry into cells.
- This is of great importance in delivering functional proteins, enzymes or other biomolecules into cells for research purposes.
- Drug Delivery: In the field of drug delivery, carrier uptake reagents are also used to improve the intracellular permeability of drugs, thereby increasing their potency. This has potential applications in drug and treatment development.
- Cell imaging studies: Some transporter capture reagents are designed by fluorescent labeling and can be used in cell imaging studies.
